v2 4 igg1 Search Results


v2 4 igg1  (Addgene inc)
93
Addgene inc v2 4 igg1
ACE2 mutant FFWF binds SARS-CoV-2 RBD with tighter affinity. (A) Expi293F cells were transfected with full-length myc-ACE2 and analyzed by flow cytometry (representative data from n = 4). Anti-myc Alexa 647 fluorescence for the full population was used to assess surface ACE2 expression levels. The fluorescence signal of bound RBD-sfGFP was measured within the blue gate to control for differences in expression levels. (B) Normalized surface expression of myc-ACE2 mutants by flow cytometry ( n = 4, mean ± SD). (C) Normalized binding of RBD-sfGFP measured by flow cytometry ( n = 4, mean ± SD). (D) Purified sACE2 2 <t>-IgG1</t> proteins were immobilized and association (time 0–60 s) and dissociation (time 60–240 s) of soluble, monomeric RBD-8h was measured by BLI. RBD-8h concentrations are indicated to the right of each response trace. Responses are gray, and fitted curves are black.
V2 4 Igg1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/v2 4 igg1/product/Addgene inc
Average 93 stars, based on 1 article reviews
v2 4 igg1 - by Bioz Stars, 2026-03
93/100 stars
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expression plasmid for sace22.v2.4-igg1  (Addgene inc)
90
Addgene inc expression plasmid for sace22.v2.4-igg1
ACE2 mutant FFWF binds SARS-CoV-2 RBD with tighter affinity. (A) Expi293F cells were transfected with full-length myc-ACE2 and analyzed by flow cytometry (representative data from n = 4). Anti-myc Alexa 647 fluorescence for the full population was used to assess surface ACE2 expression levels. The fluorescence signal of bound RBD-sfGFP was measured within the blue gate to control for differences in expression levels. (B) Normalized surface expression of myc-ACE2 mutants by flow cytometry ( n = 4, mean ± SD). (C) Normalized binding of RBD-sfGFP measured by flow cytometry ( n = 4, mean ± SD). (D) Purified sACE2 2 <t>-IgG1</t> proteins were immobilized and association (time 0–60 s) and dissociation (time 60–240 s) of soluble, monomeric RBD-8h was measured by BLI. RBD-8h concentrations are indicated to the right of each response trace. Responses are gray, and fitted curves are black.
Expression Plasmid For Sace22.V2.4 Igg1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression plasmid for sace22.v2.4-igg1/product/Addgene inc
Average 90 stars, based on 1 article reviews
expression plasmid for sace22.v2.4-igg1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


ACE2 mutant FFWF binds SARS-CoV-2 RBD with tighter affinity. (A) Expi293F cells were transfected with full-length myc-ACE2 and analyzed by flow cytometry (representative data from n = 4). Anti-myc Alexa 647 fluorescence for the full population was used to assess surface ACE2 expression levels. The fluorescence signal of bound RBD-sfGFP was measured within the blue gate to control for differences in expression levels. (B) Normalized surface expression of myc-ACE2 mutants by flow cytometry ( n = 4, mean ± SD). (C) Normalized binding of RBD-sfGFP measured by flow cytometry ( n = 4, mean ± SD). (D) Purified sACE2 2 -IgG1 proteins were immobilized and association (time 0–60 s) and dissociation (time 60–240 s) of soluble, monomeric RBD-8h was measured by BLI. RBD-8h concentrations are indicated to the right of each response trace. Responses are gray, and fitted curves are black.

Journal: Journal of Chemical Information and Modeling

Article Title: Computationally Designed ACE2 Decoy Receptor Binds SARS-CoV-2 Spike (S) Protein with Tight Nanomolar Affinity

doi: 10.1021/acs.jcim.1c00783

Figure Lengend Snippet: ACE2 mutant FFWF binds SARS-CoV-2 RBD with tighter affinity. (A) Expi293F cells were transfected with full-length myc-ACE2 and analyzed by flow cytometry (representative data from n = 4). Anti-myc Alexa 647 fluorescence for the full population was used to assess surface ACE2 expression levels. The fluorescence signal of bound RBD-sfGFP was measured within the blue gate to control for differences in expression levels. (B) Normalized surface expression of myc-ACE2 mutants by flow cytometry ( n = 4, mean ± SD). (C) Normalized binding of RBD-sfGFP measured by flow cytometry ( n = 4, mean ± SD). (D) Purified sACE2 2 -IgG1 proteins were immobilized and association (time 0–60 s) and dissociation (time 60–240 s) of soluble, monomeric RBD-8h was measured by BLI. RBD-8h concentrations are indicated to the right of each response trace. Responses are gray, and fitted curves are black.

Article Snippet: Plasmids for expression of RBD-8h (Addgene #145145), RBD-sfGFP (Addgene #141184), wild-type sACE2 2 .-IgG1 (Addgene #154104), sACE2 2 .v2-IgG1 (Addgene #154105), and sACE2 2 .v2.4-IgG1 (Addgene #154106) are previously described.

Techniques: Mutagenesis, Transfection, Flow Cytometry, Fluorescence, Expressing, Control, Binding Assay, Purification